I am currently using the PacBio portal for the purpose of denovo genome assembly of a fungal genome. Now, I have assembled genome and wanted to explore and annotate it. Could someone guide me to go further I am new to this... Thankyou
Using Genemark alone probably will give you a first good draft of your annotation. In most of case it would be enough. It depends what you plan to do next.
Genemark with proteins (Gnemark-P) or ESTs (Genemark-ET) will provide you an annotation even better.