Question: Visualizing Replicates Genome Browser
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gravatar for gregory.andrews
4 months ago by
gregory.andrews0 wrote:

How would one merge the bigwig files of biological replicates of rnaseq data for visualization in a genome browser given that different samples have different numbers of replicates (i.e, bigWigMerge is not a viable option).

rna-seq • 247 views
ADD COMMENTlink modified 4 months ago by Charles Plessy2.4k • written 4 months ago by gregory.andrews0
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gravatar for Charles Plessy
4 months ago by
Charles Plessy2.4k
Japan
Charles Plessy2.4k wrote:

The ZENBU genome browser allows you to group samples that have common metadata and visualise their expression levels as barplots with error bars. For example, visit the following view: it displays single-cell RNA-seq data data sets from 5 replicate experiments. The data was loaded with a metadata named run to distinguish the replicates. Now, click on the RNA-seq track (the one with big peaks in exons), go in the expression panel (the one under the track), open the "STATS" menu and enter run after choosing "metadata key grouping". Instead of one expression bar per sample, there will be one expression bar with error bars, for each of the 5 runs.

ADD COMMENTlink written 4 months ago by Charles Plessy2.4k
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