Question: Takara Mouse TCR a/b Profiling Sample Multiplexing
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gravatar for bioinformatics.cancer
2.5 years ago by
United States
bioinformatics.cancer220 wrote:

Hi, We would like to use the Takara Bio SMARTer Mouse TCR a/b Profiling kit for mouse TCR sequencing and are trying to figure out how many samples can be multiplexed using Illumina indexes on a single MiSeq v3 sequencer lane (PE 2x300 bp reads) to determine the cost of sequencing. Our sequencing core said that we can get 20 M reads in one lane. How many reads are required per sample for sequencing TCR a/b using this kit. Thanks, - Pankaj

rna-seq tcr takara • 815 views
ADD COMMENTlink written 2.5 years ago by bioinformatics.cancer220

This question sounds more appropriate for Takara tech support. You can use 96 indexes (384 samples) with Nextera.

ADD REPLYlink modified 2.5 years ago • written 2.5 years ago by genomax84k
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