How to do GO annotation with non-model animal

Question

[jiang_lan]

I don't know choose which tool to annotate with non-model animal, I tried the R package "AnnotationHub", however, the GO result is empty with NA, please help me! Thanks a lot.

Answer 1

[Jean-Karim Heriche]

The way to do this is to transfer annotations by orthology, i.e. use annotations from orthologs in well-annotated species.

[jiang_lan]

Thanks a lot. Could I run the GO annotation by myself?

[Jean-Karim Heriche]

What do you mean by 'run the GO annotation' ? You identify orthologs of your gene G in species X then take the GO terms associated with the orthologs of G in X and assign them to G. You could use more than one species to transfer annnotations in this way.

Answer 2

[Natasha Glover]

Hello, You can easily annotate GO functions for your own proteomes online using OMA: https://omabrowser.org/oma/functions/