Question: (Closed) Optimal aligner and variant caller
0
gravatar for pinninti1991reddy
14 months ago by
pinninti1991reddy30 wrote:

Hi

I'm analysing exome dataset, I performed the alignment and variant calling with different tools BWA-MEM, Bowtie2, Cushaw3, Novoalign. Variant calling by Freebayes and GATK-HC. How can I conclude which aligner is the best, on what parameters ? I feel, 1) Alignment percentage (no. of PE reads mapped against reference) 2) Computation time

Can you give me a strong answer.

alignment • 531 views
ADD COMMENTlink written 14 months ago by pinninti1991reddy30
1

Here you have one part of the discussion : C: BWA MEM vs BOWTIE2 , which is best?.

What are your goals ? At the end if you point some deficiencies how you will confirmed it to know which is right ?

ADD REPLYlink modified 14 months ago • written 14 months ago by Titus770
1

Hello pinninti1991reddy!

Please follow up on your questions. See C: Readgroups for a bam file ?

For this reason we have closed your question.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink modified 11 months ago • written 11 months ago by RamRS20k
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