Question: Merging PE reads before de novo RNA-Seq assembly
0
gravatar for roussine
15 months ago by
roussine10
roussine10 wrote:

Hello! can someone please give an opinion: does that make sense to merge PE reads before a de novo RNA-seq assembly (say with Trinity)? Merging tools are quite fast (like USEARCH www.drive5.com/usearch/manual/merge_pair.html) and the amount of raw data is reduced drastically. Can merged data be suitable for further assembly in a “single-ends” mode, or am I missing some theory behind it?.. Seems like it may save a lot of computations.

Thanks in advance. Leo

ADD COMMENTlink written 15 months ago by roussine10

Do you have a dataset that is largely merging? That indicates that insert sizes are much smaller that normally is the case. You could use that part of the data (which is able to merge) as single-end reads which will have sampled the entire fragment.

ADD REPLYlink written 15 months ago by genomax66k

Yes, it quite merges, a good peak at about 170 bases (~ 4/5 of all 100 bases-reads merge). Do I get right that you do not see any contras against using the merged portion for SE assemblies?

ADD REPLYlink written 15 months ago by roussine10
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1840 users visited in the last hour