I use bcftools norm to split and left-normalize variants (like the line in file). After norm the variant is split correctly and annotated, my question is can the AF tag be used to filter out AF= below 20% in the command or is there another? So in this case the first split vatiant is filtered out. Thank you :).
before bcftools norm chr4 187004216 . TC T,TT after bcftools norm chr4 187004217 187004217 C - AF=0.0305344;AO=130 chr4 187004217 187004217 C T AF=0.969466;AO=673 desired output chr4 187004217 187004217 C T AF=0.969466;AO=673