I have 60,000 sequences that I want to BLAST against the default 'Nucleotide collection (nt/nr)' database.
Is it possible to do this without setting up a standalone, local version of BLAST? (I of course have BLAST (
blast+-2.6.0) installed, but I wonder if it is possible to run the search non-locally).
blastn -db nt -query input-sequences.fasta -remote -out blast_output.out
I get quite a huge list of errors that contain strings such as: Unavailable feature GNUTLS, Failed to initialize secure session, Service not found, stack is empty, etc.
- Am I doing something wrong in my command?
- Is it faster to build a local database and search locally on my own computer for such a large number of sequences?