How to use DASCRUBBER pipeline for PacBio
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7.6 years ago
Ric ▴ 440

Hi, I was able to follow the first 4 steps of the DASCRUBBER pipeline but I am not sure how to do 5 - 8 steps.

  1. DASmap [-p] <path:db> [ <reads:FILE> |<reads:range> ... ]. What are <reads:FILE> |<reads:range>?
  2. DASrealign [-v] [-l<int:(800)>] <block1> <block2> <source:las> <target:las>. What are -l option, <block1> <block2>, source:las and target:las?
  3. REPqv <subject:db> .... Is subjet:db the source or patch db?
  4. REPtrim <subject:db> ... Is subject:db the source or patch db?

Could anyone please give an example on how to use the above commands?

Thank you in advance.

Assembly pacbio sequencing • 987 views
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