Question: 16S reads comparing
0
gravatar for luyang1005
2.4 years ago by
luyang100520
luyang100520 wrote:

Hi, community,

I have a question on the sequencing data comparison.

My reads are PAIR ENDED READS (150bp each end, merged are around 250bp) sequenced by Illumina 16S 515F-806R.

I want to analysis my reads together with EMP project data. However, I found their data are all 150bp SINGLE ENDED sequences.

May I know is there any way for me to pick the OTU together? And make them comparable?

Thanks in advance.

sequencing rna-seq next-gen • 746 views
ADD COMMENTlink modified 3 months ago by Biostar ♦♦ 20 • written 2.4 years ago by luyang100520

You should take a look at DADA2, will be useful for your comparison.

This paper will give you an idea on how the data can be comparable:

https://www.nature.com/articles/ismej2017119

ADD REPLYlink written 2.4 years ago by sridhar56100
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