Question: Hybrid de novo assembly with Illumina, Sanger and 454
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gravatar for Benni
14 months ago by
Benni30
Benni30 wrote:

I have Illumina reads of a bacterial genome and was able to assemble many parts of it. Now I also found Sanger and 454 data for the same strain. Is there a pipeline, that can handle all these data including trimming and error correction?

sequencing dna assembly • 520 views
ADD COMMENTlink modified 14 months ago by h.mon24k • written 14 months ago by Benni30
1
gravatar for h.mon
14 months ago by
h.mon24k
Brazil
h.mon24k wrote:

MIRA probably can handle every type of data and do everything for you. There are just two problems:

1) taming MIRA: it has lots of options getting the best setting can be daunting - read its wiki and user guide carefully.

2) the latest release (something like 4.9.6beta, but was really stable) seems to have disappeared, and you will have to use MIRA 4.0.2.

ADD COMMENTlink written 14 months ago by h.mon24k
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