How to accurately identify the centromere sequence of a species from draft assembly or raw reads?

Question

[bampress]

I have my plant material de novo assembled. And when we did FISH with the centromere sequence of its close relative, there was no signal. How to accurately identify its centromere sequence from the assembly or the raw reads(PE250 80X and PacBio 20X)? Are there any protocol or methods?

Answer 1

[h.mon]

Alpha-CENTAURI.

[bampress]

Many thanks. I'll try!