Question: MiSeq or HiSeq for metatranscriptome sequencing
gravatar for mariaroviscomonteiro
3 months ago by
mariaroviscomonteiro0 wrote:


I have a question. I have 6 samples in which I want to do metatranscriptomic sequencing, but my question now is whether I should use MiSeq or HiSeq technologies. I know HiSeq would give me much more reads depth but I am concerned about reads length as well. I am working with Antarctic samples so the microbial community is quite simple, however the organisms I might encounter are also not very well described, so longer reads would possibly help me further up with the assembly.

Any thoughts or experience about this?

Thank you!

sequencing rna-seq assembly • 301 views
ADD COMMENTlink written 3 months ago by mariaroviscomonteiro0

Is your goal the metagenome assembly or to asses the functional activity of the microbial community?

ADD REPLYlink written 3 months ago by Carambakaracho270
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