Question: MiSeq or HiSeq for metatranscriptome sequencing
gravatar for mariaroviscomonteiro
19 days ago by
mariaroviscomonteiro0 wrote:


I have a question. I have 6 samples in which I want to do metatranscriptomic sequencing, but my question now is whether I should use MiSeq or HiSeq technologies. I know HiSeq would give me much more reads depth but I am concerned about reads length as well. I am working with Antarctic samples so the microbial community is quite simple, however the organisms I might encounter are also not very well described, so longer reads would possibly help me further up with the assembly.

Any thoughts or experience about this?

Thank you!

sequencing rna-seq assembly • 185 views
ADD COMMENTlink written 19 days ago by mariaroviscomonteiro0

Is your goal the metagenome assembly or to asses the functional activity of the microbial community?

ADD REPLYlink written 19 days ago by Carambakaracho60
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 989 users visited in the last hour