Question: Huge jump in plasmid coverage - any idea why?
0
gravatar for predeus
9 months ago by
predeus690
Russia
predeus690 wrote:

Hello all,

I am working on some bacterial genome assemblies that have been assembled using Pacbio reads only. I realized that I can make minor improvements to them (on the order of several SNPs and indels) by aligning Illumina data from the same strain to Pacbio assembly, and polishing it with Pilon. While doing this, I discovered a very interesting phenomenon. Assembled plasmid has a huge jump in coverage right in the middle of it. The size of the region is ~ 100 bp in what seems like 85 kbp plasmid. The coverage of the plasmid is ~ 400x, while the region displayed has about 7-8000x coverage.

Any ideas about what could be causing this are greatly appreciated.

Coverage bump in the middle of the plasmid

pacbio bacteria illumina pilon • 332 views
ADD COMMENTlink written 9 months ago by predeus690
1

Maybe a repeat? - though I would expect PacBio data to have solved it. Did you blast the fragment in question? From the screenshot, it is possible to see reads with SNPs do not have the indels, and reads with the indels do not have the SNPs.

ADD REPLYlink written 9 months ago by h.mon21k
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