Question: Crispr-cas9 screen analysis mageck
0
gravatar for 11yj3312
9 months ago by
11yj33120
11yj33120 wrote:

Hi friends! Now I have a batch of paired-end sequencing data,I don't know whether mageck can handle paired-end sequencing data,I don't find appropriate parameters.What do you do with paired-end sequencing data? I try just use R1 sequencing data mapped with sgRNA library,but only about 60% reads can be mapped.What is the probable cause?How can I improve it?

sequencing mageck crispr-cas9 • 389 views
ADD COMMENTlink modified 7 months ago by Biostar ♦♦ 20 • written 9 months ago by 11yj33120

Hi there, I'm not a MAGeCK expert, but I find it difficult to understand what you're trying to achieve. I encourage you to improve your title (e.g. include MAGeCK and PE) to help the right people find your post

ADD REPLYlink written 9 months ago by Carambakaracho630
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