Hi friends! Now I have a batch of paired-end sequencing data，I don't know whether mageck can handle paired-end sequencing data,I don't find appropriate parameters.What do you do with paired-end sequencing data？ I try just use R1 sequencing data mapped with sgRNA library,but only about 60% reads can be mapped.What is the probable cause?How can I improve it?
Question: Crispr-cas9 screen analysis mageck
3 months ago by
11yj3312 • 0
11yj3312 • 0 wrote:
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