Differential Gene Analysis
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6.4 years ago
kalyanimeha ▴ 40

I am getting this error while calculating t-statistics and corresponding p-values by genefilter package. I have total 6 samples from GEO Site(GEO34747)

library(genefilter)

destats <- rowttests(exprs(gse),fac=SampleGroup)

Error in rowcoltt(x, fac, tstatOnly, 1L) :

Number of groups is 6, but must be >0 and <=2 for 'rowttests'.

Limma DGE RNA-Seq • 1.0k views
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Entering edit mode

Can you check how

exprs(gse)

and

fac

look like?

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