Question: The reads simluted with wgsim
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gravatar for 934963534
11 months ago by
93496353410
93496353410 wrote:

I am using wgsim to simulate reads from reference genome and I have a question. Is is always that the first output .fq file contians reads that has the same orientation as reference and the second contains the reverse complement orientation?

ADD COMMENTlink modified 11 months ago by Istvan Albert ♦♦ 79k • written 11 months ago by 93496353410
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gravatar for Istvan Albert
11 months ago by
Istvan Albert ♦♦ 79k
University Park, USA
Istvan Albert ♦♦ 79k wrote:

The forward and reverse complemented orientations are relative to the simulated DNA fragment - this DNA fragment may have originated from the forward or the reverse strand.

ADD COMMENTlink written 11 months ago by Istvan Albert ♦♦ 79k

So the first file may have the original orientation and the reverse orientation?

ADD REPLYlink written 10 months ago by 93496353410
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