Question: The reads simluted with wgsim
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gravatar for 934963534
15 months ago by
93496353420
93496353420 wrote:

I am using wgsim to simulate reads from reference genome and I have a question. Is is always that the first output .fq file contians reads that has the same orientation as reference and the second contains the reverse complement orientation?

ADD COMMENTlink modified 15 months ago by Istvan Albert ♦♦ 80k • written 15 months ago by 93496353420
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gravatar for Istvan Albert
15 months ago by
Istvan Albert ♦♦ 80k
University Park, USA
Istvan Albert ♦♦ 80k wrote:

The forward and reverse complemented orientations are relative to the simulated DNA fragment - this DNA fragment may have originated from the forward or the reverse strand.

ADD COMMENTlink written 15 months ago by Istvan Albert ♦♦ 80k

So the first file may have the original orientation and the reverse orientation?

ADD REPLYlink written 15 months ago by 93496353420
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