Question: Which htseq-count mode to use for handling overlaps during RNA-Seq quantitation
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gravatar for skhan
18 months ago by
skhan10
skhan10 wrote:

For counting gene expression levels (paired end stranded RNA-Seq data from mouse) using htseq-count, is there a universally accepted mode to use from the following list?

--mode union
--mode intersection-strict
--mode intersection-nonempty

To me, "union" seems like the appropriate choice but the wiki here suggests "intersection-strict" is best.

rna-seq htseq-count • 897 views
ADD COMMENTlink modified 18 months ago by lieven.sterck6.2k • written 18 months ago by skhan10
3
gravatar for lieven.sterck
18 months ago by
lieven.sterck6.2k
VIB, Ghent, Belgium
lieven.sterck6.2k wrote:

In most cases: yes, intersect-strict is the most appropriate one.

take a look here to see what the difference in behaviour of htseq is for the different modes. Depending on what you want to know/count you can switch to any of the other modes

ADD COMMENTlink written 18 months ago by lieven.sterck6.2k
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