Question: Analyzing CRISPR and RNAi genome wide screens
0
gravatar for spacemorrissey
2.4 years ago by
United States
spacemorrissey240 wrote:

I have data from genome wide CRISPR and RNAi gene knockdown screens. These were pooled experiments where genes were either knocked out with CRISPR or knocked down with RNAi, and then the surviving cells were sequenced. Tags corresponding to lethal knockouts would therefore be depleted in the resulting sequencing run. Does anyone know of a tool that will allow me to calculate a meta pvalue to combine the results of multiple experiments of this type?

rnai meta analysis crispr • 565 views
ADD COMMENTlink written 2.4 years ago by spacemorrissey240

I found this paper "Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes" https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900911/

Has anyone used this? Has anyone found anything better?

ADD REPLYlink written 2.4 years ago by spacemorrissey240
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1552 users visited in the last hour