I created a software working on abif batch files from Sanger sequencing.
I want to test it on a big amount of data to hunt for bugs, and for that i need a lot of data.
That's why i'm looking for
.ab1 files generator, or sample/anonymous
.ab1 files maybe.
I would like to get them with mutations, double, triple peaks and/or errors to be able to test my workflow properly.
I found out for now :
- We can generate Fasta + quality with
masonthat simulates sanger files but don't give the .ab1 files.
- We can Get trace data
SCFformat on NCBI and maybe convert them to
.ab1, but I couldn't find sufficient files.
- We can modify an
sangerseqRmaybe. This could work, but to get a lot of data examples, it will take quite a moment to change all and represent all cases that seams problematic...
Thank you for your time.