Hi, Probably this is a bit outside of the kinds of questions asked here but I am unable to find any suitable answer of my question so asking here. I have RNA-Seq data and I am looking for isomiRs . For alignment step I need a hairpin fasta file and a structure file (Mirna.str) . I know both of these are available at miRBase but here's the catch. I have my own lab produced annotation table for miRNA (i.e. I have fasta file for precursor miRNA) but I want to build structure files (.str) based on my miRNA database.
For example for -hsa-mir-192 structure would look like this (this is from miRBase)
Does anyone know any tool or way to do this?