Question: determination of alternate splicing events from assembled transcripts
gravatar for blooming.daisy333
21 months ago by
blooming.daisy33380 wrote:

hi all,

I want to determine alternative splicing events for my assembled transcripts (i used strigTie for transcript assembly). my genome is novel for which there is no annotation available in any of the database. i have used ASTALAVASTA software to determine alternate splicing events but this software is simply predicting the codes for splicing events for exon skipping, intron retention, alternative accepter and doner while code interpretation is too difficult to do manually and is also not providing any visualization of these events. For visualization it uses web server which is non functional for novel genome. Can anyone kindly suggest me any useful tool that could not only determine all AS events but also categorize them and provide visualization of events.

any of web based, window, linux based

many thanks

rna-seq • 427 views
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