I am learning about bisulfite sequencing and have been attempting to map some artificially generated reads back to the original genome using erne-bs5 (among others). For some reason this tool always reports mate 1 and mate 2 in each case as both being either on the forward strand or the reverse strand, in the same direction. The positions are usually correct when compared to other aligners but the orientation is wrong. What could be going on here?
Question: Mapping bisulfite-treated PE reads - why are both reads always reported to align to the same strand?
16 months ago by
adam.nunn • 0
adam.nunn • 0 wrote:
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