Entering edit mode
5.7 years ago
harry.smith
•
0
Hello,
I have read a few papers that say they identify ORFs using gffread (cufflinks), but are not very detailed in how they do this. I have gtfs generated from gffcompare (stringtie) and have looked over the -h options from gffread, but am not sure exactly how to proceed. I think it makes sense to use gffread since the files I have now come from the same workflow, but could use some help using the right syntax.
Thank you Harry
How To Extract Cds And Protein Sequences From Cufflinks Transcripts.Gtf File?
In case you are not automatically redirected, the link to the TransDecoder wiki is https://github.com/TransDecoder/TransDecoder/wiki, and the section has the same title: Starting from a genome-based transcript structure GTF file (eg. cufflinks or stringtie).
Thank you. This looks like exactly what I need.