Hello All, I am trying to install Kinannote (for eukaryotic kinase annotation from proteome) in my system, but even though the installation message says "successful", the results for the test run is just a bunch of zeros. I have read the manual a dozen times, and tried re-installing the software, but the result is the same everytime. Has anyone faced a similar situation while using Kinannote ? The dependencies to run Kinannote are hmmsearch from HMMER and blastall. I have noticed, it says "no such file or directory" for blastall, but the files exists. I am not sure what is going wrong or if anyone has any suggestions for other tools I could use to annotate the kinases in my proteome, please advice.
Running Kinannote_1.0.pl on spombe.fasta. The resulting draft kinome should be ready in about 5 minutes. Searching spombe.fasta with discovery.hmm... Scoring 0 candidate kinases using Pkinase_scoring_matrix.discTrans... BLASTing candidate kinases against KDB_20111223b... **nice: ‘/home/ncbi blast//blastall’: No such file or directory** Parsing BLAST output... Applying cutoffs to identify and classify protein kinases... Kinannote did not detect any protein kinases in the input file spombe.fasta! Done! The number of uniquely-identified sequences in spombe.fasta is 5142 The number of ePKs in spombe.fasta is 0: ePKs comprise 0.000 % of this proteome The fraction of core ePK families found in this proteome is 0.000 (1 is best) A list of hits with standardized names may be found in spombe.names A summary of this kinannote run may be found in spombe.run_summary