I conducted RT-qPCR to validate my transcriptome analysis. In my transcriptome data, the genes are expressed in Stage 1, but are not expressed in Stage 2. When I conducted qPCR, I was able to see the same pattern. However, I am facing problem when trying to analyze my data because my Cq value for Stage 2 is zero.How do I calculate my fold change for qPCR when my Cq values for Stage 2 is zero. I've used the readily available excel template and got huge positive number. Appreciate all the help I can get! Thank you!