I have aligned GRO-seq (think ChIP-seq) reads using STAR in splice-aware mode. Will my reads still align correctly if they cross an exon-intron boundary. I would think they should. This may seem like a strange question and perhaps I should have used bowtie2 or something splice-unaware. But I know STAR is pretty fast and this is a huge alignment.
Question: Can one use STAR (splice-aware) to align genomic DNA (non-spliced)
28 days ago by
goodez • 390
goodez • 390 wrote:
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