I'm reading an article in which there is a figure showing how many reads there are around the 5 prime splice site. I want to mimic this plot in my research. I am a newbie in analyzing sequencing data. I Googled but failed to find out how to define the 5 prime splice sites and then write them into a bed file, or where to download this sort of data from a public available data base.
Now what I know is that computeMatrix from deepTools can do this visualization once got the 5 splice sites region information. Can anybody help me out? Many thanks.