Smart-seq2 single-cell RNA-Seq
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3.0 years ago
wangdp123 ▴ 250

Hi there,

The data generated from Smart-seq2 single-cell RNA-Seq protocol seems to have very unbalanced gene expression values. For example, if you did the differential gene expression test, you would find out many genes are only present in one sample group. Is this normal?

I wonder if there are some specific pipelines including some normalization procedure to deal with these data?

Or alternatively, can we use the pipeline of handling the bulk RNA-Seq such as Tophat2 + Cufflink + Cuffdiff or STAR + Featurecount + DESeq2 to analyze the data from Smart-seq2 single-cell RNA-Seq protocol?

Many thanks,

Tom

RNA-Seq Smart-seq2 single-cell • 3.5k views
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Entering edit mode
3.0 years ago

That depends on what a "sample group" is? How do you analyze the data now?

I would suggest you Salmon to quantify the data and use Seurat to do the QC with a special focus on batch correction!

For DE analysis tool I suggest you take a look at Soneson et al 2018's recent benchmark.

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