Question: Missing INS in IGV?
0
gravatar for timothy.tl.ng
20 months ago by
timothy.tl.ng10 wrote:

Hi all,

I am new to this community. Nice to meet you all.

I am working on detection of SNP and INDEL of samples by aligning the read sequences (generated from nanopore sequencing) to the reference bacteria genome. After the alignment with BWA and the aligned read sorting with igvtools, I can see the alignment on IGV windows.

When I move the cursor to a position on the coverage track, I see the count of A, T, C, G, INS, and DEL. Interestingly, for example, I see 44 INS is reported on the position and I manually count the INS. I can get around 30 INS with my sight. Do I miss anything on the setting? The same happened in other mutation type (SNP and DEL). Your help will be highly appreciated.

Timothy Ng

sequencing alignment genome • 418 views
ADD COMMENTlink modified 20 months ago • written 20 months ago by timothy.tl.ng10
1

It's likely that IGV is hiding some of the alignments due to them being secondary alignments or being tagged as having quality issues. Go into the preferences in IGV and check to ensure that all alignments will be shown.

ADD REPLYlink written 20 months ago by Devon Ryan95k
1

Hi Devon,

Thank you for your infomation, I think I got it.

Timothy Ng

ADD REPLYlink written 20 months ago by timothy.tl.ng10
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 783 users visited in the last hour