Is it possible to multiplex mora than 384 samples on Novaseq 6000 for a very low pass (0.5-10x) WGS?
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5.7 years ago
Biomed 5.0k

Is it possible to multiplex mora than 384 samples on Novaseq 6000 for a very low pass (0.5-10x) WGS?

WGS Novaseq • 3.0k views
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5.7 years ago
ATpoint 84k

Yes, if you have enough barcodes there’s no technical limitation. Also see this somewhat related question:

Splitting RNA-Seq data set on two (three) flow cells

Take-Home message: You can pool as many samples as long as you have enough unique primer/index combinations.

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PerkinElmer has a set of 1536 NEXTFLEX UDI Adapters for ultra high-throughput multiplexing on Illumina flow cells.

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