Hello, I am using DAVID for the first time and struggling to understand it. I want to do a quick pathway analysis, but I am unsure how to use DAVID and how to report the results. I would really appreciate some help.
- I have a whole bunch of genes, some upregulated and some downregulated. Should I put them into the gene list together or apart?
- If I use the functional categories option, and click the "chart" button, what does this output mean? If I do this, the top hit is "disulfide bond", would it be correct to say that "the unregulated genes show enrichment for disulfide bonds"?
- If that is not correct, do I need to use the "functional annotation clustering" to analyze enrichment? My top cluster contains "disulfide bond", but also "glycoprotein" and "signal". How can I report this result?
My samples are mRNA sequenced human tissue
Thank you :):)