Question: SRA submission Error: Command based
gravatar for suren04
22 months ago by
suren0420 wrote:

Hi All, I am using command scripts for submitting my fastq.gz files to NCBI. However, I got this error message repeatedly. What could be the possible reason while submitting the fastq.gz file to NCBI SRA database?

ftp> mput *
local: 3-1a_S25_L005_R1_001.fastq.gz remote: 3-1a_S25_L005_R1_001.fastq.gz
200 PORT command successful
150 Opening BINARY mode data connection for 3-1a_S25_L005_R1_001.fastq.gz
netout: Broken pipe
421 Service not available, remote server has closed connection


rna-seq sra • 722 views
ADD COMMENTlink modified 21 months ago by Biostar ♦♦ 20 • written 22 months ago by suren0420

It is possible that you are behind a firewall that isn't allowing the incoming reverse connection from the ftp server to your client. Check with your IT folks to make sure ftp is allowed on this system.

ADD REPLYlink written 22 months ago by genomax92k
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