How much sequencing depth is required to detect a rare group of organism in environmental sample?
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5.2 years ago
Ming ▴ 110

Hello there,

I am trying to look for a rare group of organism that is low in relative abundance in my sediment samples. I have already sequenced in on dual lanes of HiSeq 2500. What are some criteria to use to see if my sequencing depth is sufficient for this group of organisms?

Thank You.

sequencing • 440 views
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