Hello everyone, I am totally new to bioinformatics and programming, but i'm sure it's the future of science and I am determined to learn .
So I will try to explain my problem, and maybe someone can help me. I have RNA-seq data, already processed and analyzed. So I have bam/bai files in a directory, and excel files with readable results of diff. expressed genes between my two conditions.
I found a program on Git Hub, name spladder https://github.com/ratschlab/spladder which should do more or less what I want, i.e find if there is differents splicing variants between my two conditions. Maybe there is better tools, if someone knows one...
The problem is that I can't even run the program, it must be something stupid but i'm totally new, and this is a nightmare for me. I'm running on Mac OS, latest version (mojave). I've installed anaconda3 on my mac, and I have a directory name anaconda3 in my harddrive, along with user, system etc.
I used git clone on my terminal to clone the repository of spladder on github. It appears in a depository name projects on my "user" page , with downloads, documents etc.
When i try to run python spladder.py in terminal, I have : :~ user$ python3 spladder.py python3: can't open file 'spladder.py': [Errno 2] No such file or directory
my PATH is user$ echo $PATH /anaconda3/bin:/miniconda3/bin:/usr/local/bin:/usr/bin:/bin:/usr/sbin:/sbin
I tried to copy/past the "projects" directory in anaconda3 file, but it changed nothing. I think there is a very simple thing I miss here...
I hope someone can help, with a lot of gratitude, Thanks