Question: (Closed) Illumina NextSeq shorter fragment size than expected
gravatar for BAGeno
2.0 years ago by
BAGeno180 wrote:


We were preparing library for NextSeq whole exome sequencing. Our starting concentration before library preparation for three samples were 27ng/µL, 37ng/µL and 42ng/µL. We pooled library and when we checked our fragment size before running on sequencer by Bioanalyzer, we saw fragment size of 239 base pair which is smaller than expected. I want to know on which step we are making mistake which resulted in smaller insert size than expected?

sequencing next-gen illumina • 326 views
ADD COMMENTlink written 2.0 years ago by BAGeno180

Hello BAGeno!

We believe that this post does not fit the main topic of this site.

Not primarily bioinformatics, try at SeqAnswers

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.


ADD REPLYlink written 2.0 years ago by WouterDeCoster45k
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