How to add biological meaning to aligned sequences?
0
0
Entering edit mode
2.7 years ago
omicfury • 0

Dear All, I'm new in the field. I want to identify microbial sequences in metatranscriptomic data deprived of rRNA. For this purpose I selected some available databases with microbial genes and aligned my reads to the databases using DIAMOND. These databases comprise of files with sequences in fasta format and annotations in .txt or .tsv format. How can I add the information about biological functions of the identified genes from annotation files to my aligned seqs?

RNA-Seq diamond alignment annotations • 645 views
ADD COMMENT
0
Entering edit mode

when you say "reads", do you mean raw reads or assembled sequences?

Can you give an example of such a database you are talking about?

ADD REPLY

Login before adding your answer.

Traffic: 2146 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6