Question: combine assemble transcripts in both genome-guided and de novo modes
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gravatar for tobytaogf
5 weeks ago by
tobytaogf0
tobytaogf0 wrote:

Hi,

I am starting doing RNA-seq analyses on Phragmites. To improve the assembly quality, I used both genome-guided assembly via close relative species S.italica and De novo assembly. I checked some paper suggesting to combine these two assemblies but without detailed description. My question is how to combine these two fasta files together (using Linux command 'cat' ? ), and how to remove the redundant after combination? What is the recommendation to do this process? Thanks for your help.

rna-seq assembly • 111 views
ADD COMMENTlink modified 5 weeks ago by Juke-342.1k • written 5 weeks ago by tobytaogf0
0
gravatar for Juke-34
5 weeks ago by
Juke-342.1k
Sweden
Juke-342.1k wrote:

EvidentalGene is one solution.

ADD COMMENTlink written 5 weeks ago by Juke-342.1k
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