Question: combine assemble transcripts in both genome-guided and de novo modes
gravatar for tobytaogf
11 months ago by
tobytaogf0 wrote:


I am starting doing RNA-seq analyses on Phragmites. To improve the assembly quality, I used both genome-guided assembly via close relative species S.italica and De novo assembly. I checked some paper suggesting to combine these two assemblies but without detailed description. My question is how to combine these two fasta files together (using Linux command 'cat' ? ), and how to remove the redundant after combination? What is the recommendation to do this process? Thanks for your help.

rna-seq assembly • 278 views
ADD COMMENTlink modified 11 months ago by Juke-343.3k • written 11 months ago by tobytaogf0
gravatar for Juke-34
11 months ago by
Juke-343.3k wrote:

EvidentalGene is one solution.

ADD COMMENTlink written 11 months ago by Juke-343.3k
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