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• Art: Simulation Tools To Generate Synthetic Next-Generation Sequencing Reads.
  ART is a set of simulation tools to generate synthetic next-generation sequencing reads. ART si...
• MutSig and hg38
  Can MutSig be run on hg38? Is it just that a new coverage file has to be generated with the updat...
• How to generate Artemis ACT comparison file of multiple sequence alignment
  Hi there, I was wondering how you would generate a comparison file for a single multiple sequenc...
• quality value with art fastq simulator
  dear all, i am trying to generate simulated fastq files from a fasta reference using [ART][1]. F...
• Any state of the art tool for reads generation from reference sequence?
  Dear Biostar Community, What is the best tool for read generation from given reference sequences...
• Calculate Short Read Mapping Sensitivity And Ppv
  Say I use ART to simulate some short reads with the purpose of evaluating the performance of a ma...
• What is the coverage when allele frequency is < 1?
  Hello All, Let's say that some whole genome sample was sequenced with a coverage of 30x. As far...
• parsing a multiple sequence alignment file
  Hi,   I have generated  clustal alignment files which show a single small aligned region of abo...
• which tools you use to generate artificial NGS?
  There is a lot of tools to generate artificial NGS  metasim maq ART RMAP Sherman dwgsim dn...
• New element for Sequence Alignment
  I have a list of sequences (12527). On this, I perform MAFFT to get the sequence alignment in clu...
• Substitution Matrix For Multiple Alignment File
  Hi all, this will be my first question here :) i have an alignement file generated using Clusta...
• Alignment to ref genome
  I have generated the genome index using default options in HISAT2. now for the alignment purpose ...
• How to parsing mugsy (.maf) alignment output to RaxML input
  Hi all, I was trying to run a phylogeny using RaxML of few bacterial genome. I have generated th...