Question: Using lima for Pacbio iso-seq data
0
gravatar for williamsbrian5064
20 months ago by
williamsbrian5064290 wrote:

Hi,

I just got back some Pacbio isoseq data. So I sequenced my sample on two different cells. Both cells had the same sample on them. I combined the two cells and have already run them through ccs. I this is my first iso-seq project. I am not sure if I need to use lima to remove the adaptors? Since the sample was the same I don't believe it was barcoded? I didn't receive a fasta file with barcodes. So I am wondering if I even need to run my data with lima? or can you run lima without a fasta file with the bar codes?

Thanks!!

ADD COMMENTlink written 20 months ago by williamsbrian5064290
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1688 users visited in the last hour