How to find where reads mapped along reference
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5.1 years ago

Hello,

I sequenced some strains of Ensifer medicae and have mapped them to the reference WSM419 using bwa. Afterwhich I sorted, reordered, etc. using picard. What I want to find out is where along the reference my reads mapped to, as I want to compare and visualize the location and percent of reads mapped between strains (Some strains mapped 85%, some 25%, I want to visualize this if possible).

While IGV is useful, I ideally want to be able to visualize whole chromosomes and plot each strain's coverage around it in circularized plots.

Thanks.

next-gen alignment sequencing • 802 views
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1
Entering edit mode
5.1 years ago

Without knowing the specific details on how you would like to have it plotted, it sounds like a job circos should be able to handle

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