Question: CHIP-SEQ - multiply aligned reads handling
gravatar for kozaleo
28 days ago by
kozaleo0 wrote:

Do I need to filter out reads that have been aligned more than once before peakcalling with MACS2?

chip-seq • 111 views
ADD COMMENTlink written 28 days ago by kozaleo0!topic/macs-announcement/ASQ3GsXQC1c

Depends on the data you are trying to process. If your regions of interest are not repeated you can filter out them before peakcalling

ADD REPLYlink written 28 days ago by Bastien Hervé4.0k
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