Question: CHIP-SEQ - multiply aligned reads handling
gravatar for kozaleo
11 months ago by
kozaleo0 wrote:

Do I need to filter out reads that have been aligned more than once before peakcalling with MACS2?

chip-seq • 243 views
ADD COMMENTlink modified 9 months ago by Biostar ♦♦ 20 • written 11 months ago by kozaleo0!topic/macs-announcement/ASQ3GsXQC1c

Depends on the data you are trying to process. If your regions of interest are not repeated you can filter out them before peakcalling

ADD REPLYlink written 11 months ago by Bastien Hervé4.5k
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