Question: Gbases, Quality control
0
gravatar for vaish01kv
3 months ago by
vaish01kv0
India
vaish01kv0 wrote:

How to calculate Gbases? I found a column exclusively for Gbases of read 1 & read 2 of 6.18 and total Gbase of 18.3 in a report. How do I interpret? How is GBases related to Quality?

ADD COMMENTlink written 3 months ago by vaish01kv0

Brief Reminder On How To Ask A Good Question

Your question needs to be significantly improved before you can get meaningful answers. Provide context of what report are you referring to? What kind of sequencing this is?

How is GBases related to Quality?

It is not related. Unless you are purely referring to getting much less sequence than what the company specifies in technical specification for a type of sequencer.

ADD REPLYlink modified 3 months ago • written 3 months ago by genomax69k

Its a Pre-alignment QC report generated using FastQC of Exome sequencing.

ADD REPLYlink modified 3 months ago • written 3 months ago by vaish01kv0

FastQC generates a qualitative QC report of sequence data. It is not possible to say anything about QC as it relates to exome at this point. You will need to do the alignments and then start looking at specific QC metrics as they relate to the regions you captured in your data.

ADD REPLYlink written 3 months ago by genomax69k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1467 users visited in the last hour