I would like to hear your opinion about this approach.
I am doing over-representation analysis on differentially expressed genes from RNA-Seq.
Instead of doing a single test, let's say, for up-regulated genes with a log2FC cut off of x, I am doing different test within different cut off intervals. For example, one test for genes up with log2FC between 0 and 0.5, then one for the genes up with log2FC between 0.5 and 1 and so on. And then separately for negative fold changes.
Later I do not mix results but I just compare the terms found enriched with different cut off intervals.
This way I check if genes that are significantly up or down regulated with similar intensity are enriched for specific terms/ontologies that might not be spotted if considering a single cut off value.
Hope I was clear