I am trying to run psicoffee mode with t_coffee on locally installed blast with a local database. I have installed latest blast+ on my system. It works fine for regular t_coffee.
I am following the TCoffee Documentation after installing TCoffee on my local system.
However, I am stuck with the following errors
When I run "t_coffee file.fasta -mode psicoffee -blast_server=LOCAL -protein_db=home/user/db", I get the following error
--ERROR: # [FATAL:T-COFFEE] The Program legacy_blast.pl Needed by T-COFFEE Could not be found If legacy_blast.pl is installed on your system: -Make sure legacy_blast.pl is in your $path:
When I run "t_coffee file.fasta -mode psicoffee -blast_server=/usr/bin/blastp", I get the following error
--WARNING: Impossible to find BLAST Templates Check that your blast server is properly installed [See
I have added path to my local blast. Also tried to give path to legacy_blast.pl. I have also tried to use the flags -blast_server and -protein_db without "=" as per T-Coffee documentation (pdf page 65 or document page 55). I am also following the documentation here.
I am lost at this point. I would be very glad if someone could help me out. Please let me know if you need more info. Thank you.