When I use the estimate_richness in function in phyloseq in R, the given Chao1 values are identical to our observed richness. There is not a single point of difference.
I was wondering what this might mean? Is our sampling depth truly perfect? Or is there some problem that this is indicative of.
I haven't normalized or trimmed the data in any way prior to performing this analysis.
Thank you, please let me know if you need more info.