I performed MinION sequencing run using MinKNOW software,and 4 days after sequencing finish it had only 24% of bases called. So, I decided to do base calling on more powerful computer with using Guppy. But I am not sure, which data I should take as input? Input file format for Guppy is .fast5.
I suppose that .fast5 data are generated after base calling, and if I continue base calling via MinKNOW, it will work about 2 weeks till completion.
I also found "queued_reads" folder in my data, and it contains 512 folders (which I suppose come from 512 channels on MinION flowcell), and these folders contain files of .raw format. Is there any software to continue base calling based on these .raw files as input? Or the only way is to wait until 100% .fast5 files are generated?
Thank you, Anastasiia