Question: Isoform level RNA-seq analyses
gravatar for BPors
14 months ago by
BPors40 wrote:


I am considering to filter my transcripts based on expression, in that case, what are the options to choose the highly expressed isoform of each gene in a count matrix ( for example, salmon or FeatureCounts)?

I found out that trinity has an option of isoform level filtering ( via its ), but I was wondering, if there are other options you know of & trust in your analyses?

Thank you!

isoform rna-seq • 270 views
ADD COMMENTlink modified 14 months ago • written 14 months ago by BPors40

What is the analysis goal? Why do you want to filter?

ADD REPLYlink written 14 months ago by ATpoint39k

I would like to keep the isoforms which are always being expressed regardless of the condition, so that I can use them in other analyses, for ex. checking how translation is effected etc.

ADD REPLYlink modified 14 months ago • written 14 months ago by BPors40
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 777 users visited in the last hour