I'm looking to use FASTX to trim off 15 bases at 3’ end of read 1 and 15 bases at 5’ of read 2. But when I look at the FASTX command, it's not entirely clear to me on how to go about it. The two options that would be used are:
[-f N] = First base to keep. Default is 1 (=first base). [-l N] = Last base to keep. Default is entire read.
Is there a way to tell FASTQ with the -l option to remove the last 15 bases for read one? And for read 2, I would just use -f 16, correct? As this would start with the 16th base pair.