Question

value of FLAG for unpaired read

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4.6 years ago

gabrielerosso13 • 0

Hi,

in case a read is unpaired, the value of the first bit of FLAG is set to "0" or "1"?

thanks at all

unpaired read flag • 661 views

ADD COMMENT • link updated 4.5 years ago by Biostar 20 • written 4.6 years ago by gabrielerosso13 • 0

score 0 · Answer 1 · 2019-09-27

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4.6 years ago

swbarnes2 14k

Zero. With single end data, you see a lot of flags values of 0,4,and 16, 256.

ADD COMMENT • link 4.6 years ago by swbarnes2 14k

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So, unpaired reads are processed like single read?

ADD REPLY • link 4.6 years ago by gabrielerosso13 • 0